Biotechnology

Front

Southern blot is the technique for _____________ blot

Back

DNA

Front

_________ enzyme is used in the elongation/ extension of DNA fragment in PCR reaction

Back

Revers transcriptase

Front

_________ media is used for hybridoma cell culture. Options: HAT, CHO-S, Feed - 220, Leibovitz L-15

Back

HAT

Front

In Southern blotting, _________ type of gel is used for the separation of molecular band.

Back

Agarose

Front

The technique PCR was discovered by the scientist __________ in 1985.

Back

Kary Mullis

Front

In PCR, denaturation of DNA takes place at ________ ⁰C temperature.

Back

94⁰C

Front

The enzyme DNA polymerase used in PCR reaction is isolated from which bacteria?

Back

Thermus aquaticus

Front

In PCR reaction, how much kinds of nucleotide are used?

Back

4

Front

In Southern blotting, ___________ type paper is used for blot transfer

Back

Nitrocellulose membrane or Nylon membrane filter

Front

Who developed the procedure for DNA transfer or for the DNA blot in 1975?

Back

E. M. Southern