Bacterial Transformation

Front

What is competency for a cell?

Back

Change in the structure and permeability of the cell membrane.

Front

How might a plasmid contribute to a selective advantage for a bacterial cell?

Back

Could provide a selective advantage for that cell.

Front

What is transformation?

Back

The insertion of a foreign plasmid into a bacterial cell which results in new acquired traits.

Front

Cells are made competent by:

Back

A process that uses calcium chloride.

Front

Heat shock

Back

Enlarges the pores of the cell.

Front

The exogenous DNA used in bacterial transformation can be: mRNA molecule, rDNA, engineered plasmid, red fluorescent protein.

Back

engineered plasmid

Front

LB containing ampicillin (2nd plate) is inoculated with the E. coli bacteria that contains ampicillin resistant gene. The bacteria will:

Back

Grow on the agar plate

Front

Which of the following statement is true concerning transformation?

Back

Transformation efficiencies are a way to determine how many cells were transformed per microgram of plasmid DNA used.

Front

What gene is present in the engineered plasmid? Options: kanamycin resistant gene, ampicillin resistant gene, tetracycline resistant gene, No plasmid is necessary to grow on plate 3.

Back

tetracycline resistant gene

Front

Why would group 4 see growth without RFP in the third plate?

Back

The bacteria cells transformed, but did not incorporate the plasmid containing the RFP gene.